Human IL-18/IL-1F4 Propeptide Phycoerythrin MAb (74801) (100 TESTS)【科瑞奥博】

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Human IL-18/IL-1F4 Propeptide Phycoerythrin MAb (74801) (100 TESTS)

货号： IC646P 基本售价： 4382.2 元规格： 100 Tests

产品信息

概述
货号	IC646P
别名	Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18
全称	Interleukin 18
反应种属	Human
应用	Intracellular Staining by Flow Cytometry
目标/特异性	Detects the pro region of human IL-18/IL-1F4 in direct ELISAs and Western blots. Does not cross‑react with recombinant human IL-18, recombinant mouse IL-18 or recombinant rat IL-18.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells Authors: Y Hu, J Wang, H Zhang, H Xie, W Song, Q Jiang, N Zhao, S He Mediators Inflamm., 2017;2017(0):3090782. Species: Human Sample Type: Whole Cells Application: Flow Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He J. Cell. Mol. Med., 2017;0(0):. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human IL‑18/IL‑1F4 Accession # Q14116
生物活性	Human
标记	Phycoerythrin
背景	Pro-IL-18 (pro-Interleukin 18; also known as pro-IGIF and pro-IL-1 gamma ) is a 24 kDa member of the IL-1 family of molecules. It is widely expressed, being produced by keratinocytes, intestinal epithelium, T cells, macrophages and osteoblasts. Human Pro-IL-18 is 193 amino acids (aa) in length. Although mature IL-18 induces IFN-gamma secretion by NK and T cells, Pro-IL-18 appears to have little intrinsic activity. Generally, active IL-18 is considered to arise from caspase-1 cleavage of Pro-IL-18 between Asp36-Tyr37. This generates an 18 kDa mature C-terminal fragment, and a 4 kDa (predicted) N-terminal prosegment that runs at 6 kDa in SDS-PAGE. Other proteases are known to process Pro-IL-18. Caspase-3 cleavage after Asp68 generates an inactive 14 kDa mature segment, Merpin beta -subunit cleavage after Asn52 generates a marginally active 17 kDa mature segment, while parasite Cys protease cleavage after Val47 generates an inactive 17 kDa mature molecule. One splice variant shows a deletion of aa 27-30. Over aa 2-36, human Pro-IL-18 shares 63% aa identity with mouse Pro-IL-18.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Angiogenesis Group 1 ILCs IL-1 Family Inflammasomes: Intracellular Regulators of Pathogen Interactive Pathway: IL-1 Family Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Mini-review: Interleukin 18 (IL-18) Multiple Sclerosis and Myelin Related Molecules Neuroinflammation NOD-like Receptors and the Inflammasome T Cell Cytokine Signaling Th1 Cells The Nlrp3 Inflammasome & IL-18 Regulate Intestinal Homeostasis

参考图片

Detection of IL‑18/IL‑1F4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (upper panel) or treated with LPS for 24 hours (lower panel) were stained with Mouse Anti-Human IL‑18/IL‑1F4 PE‑conjugated Monoclonal Antibody (Catalog # IC646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.