货号 | IC654R-100UG |
别名 | CXCL5; CXCL5/ENA-78; ENA78; ENA-78 |
应用 | Intracellular Staining by Flow Cytometry |
目标/特异性 | Detects human CXCL5/ENA-78 in ELISAs. Does not cross-react with recombinant human CXCL1, 2, or 3. |
使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 6374 (Human) |
纯化方式 | Protein A or G purified from ascites |
免疫原 | E. coli-derived recombinant human CXCL5/ENA-78 Ala37-Asn114 Accession # P42830 |
生物活性 | Human |
标记 | Alexa Fluor 647 |
背景 | CXCL5, also known as ENA-78, is a CXC chemokine that plays a role in inflammatory responses and angiogenesis. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of CXCL5/ENA-78 in Human PBMC by FlowCytometry Human PBMC treated with LPS were stainedwith Mouse Anti-HumanCXCL5/ENA-78 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog# IC654R, filled histogram) or isotype control antibody (Catalog # IC002R,open histogram). To facilitate intracellular staining, cells were fixed withFlow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for Staining Intracellular Molecules. |