| 货号 | FAB74701S-100UG |
| 别名 | A8; BB18; CD100 antigen; CD100; CD100M-sema-G; COLL-4; FLJ33485; FLJ34282; FLJ39737; FLJ46484; immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D; MGC169138; MGC169141; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D; SEMA4D; SEMAJ; Semaphorin 4D; semaphorin-4D |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human Semaphorin 4D/CD100 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinanthuman Semaphorin 4B, 4C, 4G, or recombinant mouse Semaphorin 4D is observed. In Western blots, no cross-reactivity withrhSemaphorin 4A, 6A, or 6C is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10507 (Human); 20354 (Mouse); 306790 (Rat) |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Semaphorin 4D/CD100 Met22-Arg734 Accession # Q92854 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 750 |
| 背景 | Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Semaphorin 4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Semaphorin 4D shares 87% and 93% aa identity with human and rat Semaphorin 4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Semaphorin 4D binds CD72 on B and dendritic cells and Plexin B1 elsewhere. In developing neurons, Semaphorin 4D assists in guidance. Semaphorin 4D is proangiogenic when expressed by tumor‑associated macrophages. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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