| 货号 | FAB099V-100UG |
| 别名 | BLT1; chemokine receptor-like 1; CMKRL1; Leukotriene B4 R1; leukotriene B4 receptor; Leukotriene B4R1; LTB4R; LTB4R1; LTBR1; P2Y purinoceptor 7; purinergic receptor P2Y, G-protein coupled, 7 | 全称 | Leukotriene B4 Receptor 1 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Recognizes human Leukotriene B4 R1 (Pettersson, A. et al. (2000) Biochem. Biophys. Res. Commun. 279:520). |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1241 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | HeLa cervical epithelial carcinoma cell line transfected with human Leukotriene B4 R1 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 405 |
| 背景 | Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human Leukotriene B4 R1 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB099V) or (B) Mouse IgG1 Alexa Fluor® 405 Isotype Control (Catalog # IC002V). View our protocol for Staining Membrane-associated Proteins. |
