| 货号 | FAB619P |
| 别名 | EC 2.7.10; EC 2.7.10.1; JTK14soluble TIE1 variant 3; receptor tyrosine kinase; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 5; TIEsoluble TIE1 variant 4; tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; tyrosine-protein kinase receptor Tie-1 | 全称 | Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Tie‑2 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7075 (Human); 21846 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Tie-1 Ala22-Gln760 Accession # P35590 |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains, flanking three epidermal growth factor (EGF)-like domains followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human Tie-1 cDNA encodes a 1138 amino acid (aa) residue precursor protein with a 24 residue putative signal peptide, a 735 residue extracellular domain, a 25 residue transmembrane domain, and a 354 residue cytoplasmic domain. Ligands which bind and activate Tie-1 have not been identified. Based on gene-targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Tie‑1 in Human Umbilical Vein Endothelial Cells (HUVEC) by Flow Cytometry. Human umbilical vein endothelial cells were stained with Goat Anti-Human Tie‑1 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB619P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
