Human IL-1 beta/IL-1F2 Phycoerythrin MAb (Clone 8516) (100 TESTS)【科瑞奥博】

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Human IL-1 beta/IL-1F2 Phycoerythrin MAb (Clone 8516) (100 TESTS)

货号： IC201P 基本售价： 4243.4 元规格： 100 Tests

产品信息

概述
货号	IC201P
别名	catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and recombinant porcine IL‑1 beta and no cross-reactivity with recombinant rat (rr) IL‑1 beta, rmIL‑1 alpha, recombinant human IL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli. Authors: Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T J. Immunol., 2011;186(12):6701-9. Species: Human Sample Type: Whole Cells Application: Flow A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients. Authors: Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB Cancer Res., 2007;67(20):9630-6. Species: Human Sample Type: Whole Cells Application: Flow A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC). Authors: Novak N, Kraft S, Haberstok J, Geiger E, Allam P, Bieber T J. Invest. Dermatol., 2002;119(4):842-9. Species: Human Sample Type: Whole Cells Application: Flow Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production. Authors: Wang L, Das H, Kamath A, Li L, Bukowski JF J. Immunol., 2002;168(10):4889-96. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IL-1 beta /IL-1F2 aa 117-269 Accession # P01584
生物活性	Human
标记	Phycoerythrin
背景	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21. March, C.J. et al. (1985) Nature 315:641. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. USA 81:7907. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

参考图片

Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes stimulated with LPS were stained with Mouse Anti-Human CD14 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human IL‑1 beta /IL‑1F2 PE‑conjugated Monoclonal Antibody (Catalog # IC201P) or (B) Mouse IgG₁ Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.