Human CD27/TNFRSF7 Allophycocyanin MAb (Clone 57703) (100 TESTS)【科瑞奥博】

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Human CD27/TNFRSF7 Allophycocyanin MAb (Clone 57703) (100 TESTS)

货号： FAB382A 基本售价： 4243.4 元规格： 100 Tests

产品信息

概述
货号	FAB382A
别名	CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
反应种属	Human
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human CD27 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) 4‑1BB, rhBAFF R, recombinant mouse (rm) CD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rmOX40, rhRANK, rhTAJ, or rhTNF RI.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	939 (Human); 21940 (Mouse); 500318 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD27 Thr21-Ile192 Accession # P26842
生物活性	Human
标记	Allophycocyanin
背景	Human CD27 is a 50-55 kDa lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes as well as NK cells, B cells, and CD4⁺ and CD8⁺ T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4⁺ compartment, it is preferentially expressed on CD45RA⁺ cells. In contrast, it is preferentially expressed on CD45RO⁺ cells in the CD8⁺ compartment. CD27 also appears to be a potential marker for memory B cells. It exists as both a disulfide-linked dimer on the cell surface and as a soluble 32 kDa protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 19 aa signal, a 173 aa extracellular domain, a 21 aa transmembrane domain, and a 48 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and multiple activated leukocytes including NK cells, CD4⁺ and CD8⁺ T cells, and gamma δ T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses. Over aa 21-192, human and mouse CD27 share 60% aa sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Markers Cancer Stem Cell Markers Co-stimulatory and Co-inhibitory Molecules gamma delta T Cells Human CD Marker Products Isolation, Culture, and Identification of Natural Killer Cells Regulation of Apoptosis by TNF Superfamily Members Regulation of B Cell Activation and Homeostasis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members Regulatory T Cells (Tregs) TNF Superfamily TNF Superfamily Receptors

参考图片

Detection of CD27/TNFRSF7 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD27/TNFRSF7 APC‑conjugated Monoclonal Antibody (Catalog # FAB382A) or (B) Mouse IgG₁ Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.