Human N-Cadherin Propeptide Phycoerythrin MAb (Clone 401408) (100 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human N-Cadherin Propeptide Phycoerythrin MAb (Clone 401408) (100 TESTS)

货号： IC1388P 基本售价： 4243.4 元规格： 100 Tests

产品信息

概述
货号	IC1388P
别名	cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; CD325 antigen; CD325; CDH2; CDHNcalcium-dependent adhesion protein, neuronal; CDw325; N-cadherin; NCADN-cadherin 1; Neural cadherin; neural-cadherin
全称	Neural Cadherin
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human N-Cadherin propeptide in direct ELISAs. Shows no cross-reactivity with recombinant human (rh) Cadherin-4, -6, -8, -11, -12, -13, -17, rhE-Cadherin, rhP-Cadherin and rhVE-Cadherin.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1000 (Human); 12558 (Mouse); 83501 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human N-Cadherin propeptide Pro26-Lys159 Accession # P10922
生物活性	Human
标记	Phycoerythrin
背景	N‑Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130‑135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N‑Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN‑Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15‑20 kDa prodomain (aa 26‑159) is cleaved by proprotein convertase, and the mature molecule is transported to the surface. Alternatively, on neurons, proN‑Cadherin may first appear on the surface, with cleavage occurring at the time of synaptogenesis. Cleavage appears necessary for homophilic interaction as presence of the prodomain is suggested to negatively regulate oligomer formation. Over the entire prodomain, the human N‑Cadherin proregion shares 87% aa identity with the mouse N‑Cadherin proregion.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adhesion Molecules Cadherin Superfamily Early Mesodermal Lineage Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Human CD Marker Products ICC / IHC Images: N-Cadherin Mesenchymal Cells and EMT Mesenchymal Stem Cell Markers Mini-review: Adhesion Molecules II Neural Stem Cell Markers Regulation of VE-Cadherin and VEGF R2 by VEGF Synaptic Adhesion Molecules Synaptic Proteins

参考图片

Detection of N‑Cadherin Propeptide in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human N‑Cadherin Propeptide PE‑conjugated Monoclonal Antibody (Catalog # IC1388P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.