| 货号 | FAB663P |
| 别名 | beta; CD121b antigen; CD121b; IL-1 R beta; IL1R2; IL1RBCD121 antigen-like family member B; IL-1R-beta; IL-1RT2; IL-1RT-2; interleukin 1 receptor, type II; Interleukin-1 receptor beta; MGC47725 | 全称 | Interleukin 1 Receptor II |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human IL-1 RII in direct ELSAs and Western blots. When used in combination with the biotinylated human IL-1 RII affinity purified polyclonal detection antibody (Catalog # BAF263) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) IL-1ra, rhIL-1 RI, recombinant mouse IL-1ra, or recombinant rat IL-1ra. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7850 (Human); 16178 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑1 RII Phe14-Glu343 (Ser56Gly and Glu297Gly) Accession # P27930 |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta, but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑1 RII in Human PBMCs stimulated to induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs), stimulated to induce Regulatory T Cells (Tregs), were treated with 10 ug/mL Anti-CD3, 5 ug/mL Anti-CD28, 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) for 48 hours and stained with Rat Anti-Human LRRC32/GARP APC‑conjugated Monoclonal Antibody and either (A) Mouse Anti-Human IL‑1 RII PE‑conjugated Monoclonal Antibody (Catalog # FAB663P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). Cells were gated on CD4. View our protocol for Staining Membrane-associated Proteins. |
