Human MMP-2 Phycoerythrin MAb (Clone 1A10) (100 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human MMP-2 Phycoerythrin MAb (Clone 1A10) (100 TESTS)

货号： IC9023P 基本售价： 4243.4 元规格： 100 Tests

产品信息

概述
货号	IC9023P
别名	72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
全称	Matrix Metalloproteinase 2
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects the pro and active forms of human MMP-2 in Western blots. In dot blots, no cross-reactivity with recombinant human MMP-1, -3, -7, -8, -9, -10, -12, or -13 is observed.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4313 (Human); 17390 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. MMP9 integrates multiple immunoregulatory pathways that discriminate high suppressive activity of human mesenchymal stem cells Authors: C Lavini-Ram, HM Silva, A Soares-Sch, SM Monteiro, LR Ferreira, AP Pacanaro, S Gomes, J Batista, K Faé, J Kalil, V Coelho Sci Rep, 2017;7(1):874. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human MMP-2 Accession # P08253
生物活性	Human
标记	Phycoerythrin
背景	Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases that have a combined ability to degrade all the components of the extracellular matrix. MMP‑2 (Gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagen, as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP‑1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP‑2 has been shown to be associated with many connective tissue cells, as well as neutrophils, macrophages and monocytes. Structurally, MMP‑2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.
运输条件	Blue Ice
存放说明	4℃
参考文献	APP Cleavage and Amyloid-beta Degradation Cancer Biomarkers Collagen Modifying Enzymes Extracellular Matrix Remodeling and Cell Migration During EMT Gametes and Fertilization HIF Transcription Factors ICC / IHC Images: MMP-2 Inflammatory Mediators Mesenchymal Cells and EMT Metalloproteases and Regulators MMP Modulation of Cytokines MMP-12 in Smoking-Related Pulmonary Disease MMPs, TIMPs and Related Molecules New Tools: Active MMP Activity Assays OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression Platelets Preeclampsia

参考图片

Detection of MMP‑2 in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human MMP‑2 PE‑conjugated Monoclonal Antibody (Catalog # IC9023P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.