Human Nestin Phycoerythrin MAb (Clone 196908) (100 TESTS)【科瑞奥博】

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Human Nestin Phycoerythrin MAb (Clone 196908) (100 TESTS)

货号： IC1259P 基本售价： 4243.4 元规格： 100 Tests

产品信息

概述
货号	IC1259P
别名	FLJ21841; NES; nestin
反应种属	Human
应用	Intracellular Staining by Flow Cytometry
目标/特异性	Detects human Nestin.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10763 (Human); 18008 (Mouse); 25491 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Phenotypic diversity of patient-derived melanoma populations in stem cell medium. Authors: Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M Lab Invest, 2015;95(6):672-83. Species: Human Sample Type: Whole Cells Application: Flow Expression of the stem cell markers nestin and CD133 on circulating melanoma cells. Authors: Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U J. Invest. Dermatol., 2011;131(2):487-94. Species: Human Sample Type: Whole Cells Application: Flow MDR1 expression identifies human melanoma stem cells. Authors: Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G Biochem. Biophys. Res. Commun., 2008;368(4):930-6. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NS0 mouse myeloma cell line transfected with human Nestin
生物活性	Human
标记	Phycoerythrin
背景	Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310. Lendahl, U. et al. (1990) Cell 60:585. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144. Tohyama, T. et. al. (1992) Lab. Invest. 66:303. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720. Frederiksen, K. et al. (1988) Neuron 1:439. Cattaneo, E. et al. (1990) Nature 347:762. Reynolds, B.A. and S. Weiss (1992) Science 255:1707. Rietze, R.L. et al. (2001) Nature 412:736. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383. Zulewski, H. et al. (2001) Diabetes 50:521. Lumelsky, N. et al. (2001) Science 292:1389. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670. Shih, C.C. et al. (2001) Blood 98:2412.

参考图片

Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either untreated (dark orange filled histogram) or treated with 5 μM Retinoic Acid for 60 days (light orange filled histogram) was stained with Mouse Anti-Human Nestin PE‑conjugated Monoclonal Antibody (Catalog # IC1259P) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.