| 货号 | IC11542P |
| 别名 | AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3 |
| 反应种属 | Human |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human Galectin-3 in direct ELISAs. In direct ELISAs, 100% cross-reactivitywith recombinant mouse Galectin-3 is observed and no cross-reactivity withrecombinant human (rh) Galectin-8 or rhGalectin-9 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3958 (Human); 16854 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Galectin-3 Ala2-Ile250 Accession # P17931 |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | Galectin-3, also known as Mac-2, L29, CBP35 and IgE Binding Protein, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their Carbohydrate Recognition Domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts where it forms homodimers, or heterodimers, likely with DMBT1. It is reported to complex with Integrins alpha3 and beta1. Galectin-3 is known to circulate and show elevation in inflammatory disorders. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Galectin‑3 in Human Blood Monocytes by Flow Cytometry. LPS-treated peripheral blood monocytes were stained with Mouse Anti-Human Galectin‑3 PE‑conjugated Monoclonal Antibody (Catalog # IC11542P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
