| 货号 | IC1419A |
| 别名 | BGLAP; BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein; OC; OCN; osteocalcin | 全称 | Bone gamma-Carboxyglutamate [gla] Protein |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human Osteocalcin in direct ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 632 (Human); 12096 (Mouse); 25295 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dysregulation of ossification related microRNAs in circulating osteogenic progenitor cells obtained from patients with aortic stenosis | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human Osteocalcin synthetic peptide YLYQWLGAPVPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV Accession # P02818 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | Osteocalcin, also known as Bone gamma -Carboxyglutamic Acid Protein, is a secreted protein whose expression is restricted to cells of the osteoblast lineage (1). It has been frequently used as a marker for osteoblast lineage cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Osteocalcin in Saos‑2 Human Cell Line by Flow Cytometry. Saos‑2 human osteosarcoma cell line was stained with Mouse Anti-Human Osteocalcin APC‑conjugated Monoclonal Antibody (Catalog # IC1419A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. | |
Detection of Osteocalcin in Human Osteoblasts by Flow Cytometry. Human osteoblasts were stained with Mouse Anti-Human Osteocalcin APC‑conjugated Monoclonal Antibody (Catalog # IC1419A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
