Human TACE/ADAM17 Phycoerythrin MAb (Clone 111633) (100 TESTS)【科瑞奥博】

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Human TACE/ADAM17 Phycoerythrin MAb (Clone 111633) (100 TESTS)

货号： FAB9301P 基本售价： 4243.4 元规格： 100 Tests

产品信息

概述
货号	FAB9301P
别名	ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme
全称	TNF-alpha Converting Enzyme
反应种属	Human
应用	Flow Cytometry
目标/特异性	Detects the ectodomain of human TACE/ADAM17 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15 and recombinant mouse ADAM10 is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6868 (Human); 11491 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Unlike for human monocytes after LPS activation, release of TNF-ÃŽÂ± by THP-1 cells is produced by a TACE catalytically different from constitutive TACE. Authors: Moreira-Tabaka H, Peluso J, Vonesch JL, Hentsch D, Kessler P, Reimund JM, Dumont S, Muller CD PLoS ONE, 2012;7(3):e34184. Species: Human Sample Type: Whole Cells Application: Flow Increased activation of fibrocytes in patients with chronic obstructive asthma through an epidermal growth factor receptor-dependent pathway. Authors: Wang CH, Huang CD, Lin HC, Huang TT, Lee KY, Lo YL, Lin SM, Chung KF, Kuo HP J. Allergy Clin. Immunol., 2012;129(5):1367-76. Species: Human Sample Type: Whole Cells Application: Flow Microparticles of human atherosclerotic plaques enhance the shedding of the tumor necrosis factor-alpha converting enzyme/ADAM17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1. Authors: Canault M, Leroyer AS, Peiretti F, Leseche G, Tedgui A, Bonardo B, Alessi MC, Boulanger CM, Nalbone G Am. J. Pathol., 2007;171(5):1713-23. Species: Human Sample Type: Tissue Homogenates Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17 Pro18-Asn671 Accession # P78536
生物活性	Human
标记	Phycoerythrin
背景	TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of Tumor Necrosis Factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.
运输条件	Blue Ice
存放说明	4℃
参考文献	Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.

参考图片

Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain PE‑conjugated Monoclonal Antibody (Catalog # FAB9301P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.