货号 | FAB7227G |
别名 | CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 |
反应种属 | Human |
应用 | Flow Cytometry(5 µL/106cells) Immunocytochemistry(10 µg/mL) |
目标/特异性 | Detects human CD34 in direct ELISAs. |
使用方法 | Flow Cytometry: 5 µL/106cells Immunocytochemistry: 10 µg/mL |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Human endothelial vesicles |
生物活性 | Human |
标记 | Alexa Fluor 488 |
背景 | The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD45 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1430N) and either (A) Mouse Anti-Human CD34 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7227G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins. | |
CD34 in KG-1 Human Cell Line. CD34 was detected in immersion fixed KG-1 human acute myelogenous leukemia cell line using Mouse Anti-Human CD34 AlexaFluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7227G, green) at 10 μg/mL for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |