货号 | FAB4225A |
别名 | B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A2; S7 | 全称 | Membrane-spanning 4-domains, Subfamily A, Member 1 |
反应种属 | Human |
应用 | Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects human MS4A1/CD20. Stains human MS4A1/CD20 transfectants but not irrelevant transfectants. |
使用方法 | Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 931 (Human); 12482 (Mouse); 25316 (Rat) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | NS0 mouse myeloma cell line transfected with human MS4A1/CD20 Met1-Pro297 Accession # P11836 |
生物活性 | Human |
标记 | Allophycocyanin |
背景 | CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanning protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa identity between human and mouse CD20. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of MS4A1/CD20 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human MS4A1/CD20 APC‑conjugated Monoclonal Antibody (Catalog # FAB4225A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins. | |
Detection of MS4A1/CD20 in HEK293 Human Cell Line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP was stained with either (A) Mouse Anti-Human MS4A1/CD20 APC‑conjugated Monoclonal Antibody (Catalog # FAB4225A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins. |