Human CD300f/LMIR3 Alexa Fluor 488 MAb (Clone 234903) (100 TESTS)【科瑞奥博】

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Human CD300f/LMIR3 Alexa Fluor 488 MAb (Clone 234903) (100 TESTS)

货号： FAB2774G 基本售价： 4094.7 元规格： 100 Tests

产品信息

概述
货号	FAB2774G
别名	CMRF35-like molecule 1; CD300 molecule-like family member f; CD300f; CD300LF; CLIM1; CLM1; CLM-1; DIgR2; IGSF13; IREM1; IREM-1; LMIR3; NKIR; Pigr3
全称	Leukocyte Mono Ig-like Receptor 3
反应种属	Human
应用	Flow Cytometry(5 µL/10⁶cells)
目标/特异性	Detects human CD300f/LMIR3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 4, 5, 6, or recombinant mouse LMIR3 is observed.
使用方法	Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	146722 (Human); 246746 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Y3 rat myeloid cell line transfected with human CD300f/LMIR3
生物活性	Human
标记	Alexa Fluor 488
背景	CD300f, also known as CD300LF, LMIR3, IREM-1, CLM-1, IgSF13, DIgR1, and MAIR-V, is a 50‑60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human CD300f consists of a 137 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 113 aa cytoplasmic domain that contains multiple immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or ITIM-like sequences (2, 3). Alternate splicing generates additional isoforms that carry substituted C-terminal tails of varying lengths and sequences following the ECD or transmembrane segment (3). Within the ECD, human CD300f shares 43% aa sequence identity with mouse and rat CD300f. CD300f is expressed on the surface of dendritic cells, monocytes, granulocytes, and mast cells as well as on acute myeloid leukemia (AML) blasts (2-4). Pervanadate treatment or antibody crosslinking of CD300f induces phosphorylation of tyrosine residues in the cytoplasmic domain and the subsequent recruitment of phosphatases SHIP, SHP-1, SHP-2, and the p85 alpha subunit of PI3K (2, 3, 5, 6). CD300f ligation can induce cell death and inhibit signaling through multiple receptors including Fc epsilon RI, LMIR4, SCF R, TLR2, TLR3, and TLR9 (3-8). In contrast, it enhances TLR4-mediated signaling/cytokine production in mast cells through association with the activating signaling protein FcR gamma (5). In mouse, a splice variant of CD300f (known as DIgR2, with a 7 aa insertion in the ECD) inhibits CD4⁺ T cell activation and in vivo Th1 and CTL responses (9). CD300f is upregulated on monocytes surrounding experimentally-induced spinal cord demyelination and functions as a negative regulator of inflammation in the CNS (10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Clark, G.J. et al. (2009) Trends Immunol. 30:209. Sui, L. et al. (2004) Biochem. Biophys. Res. Commun. 319:920. Alvarez-Errico, D. et al. (2004) Eur. J. Immunol. 34:3690. Korver, W. et al. (2009) Leukemia 23:1587. Izawa, K. et al. (2009) J. Immunol. 183:925. Alvarez-Errico, D. et al. (2007) J. Immunol. 178:808. Can, I. et al. (2008) J. Immunol. 180:207. Izawa, K. et al. (2007) J. Biol. Chem. 282:17997. Shi, L. et al. (2006) Blood 108:2678. Xi, H. et al. (2010) J. Exp. Med. 207:7.

参考图片

Detection of CD300f/LMIR3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human CD300f/LMIR3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB2774G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). View our protocol for Staining Membrane-associated Proteins.