Human CD6 Alexa Fluor 488 Affinity Purified PAb (100 TESTS)【科瑞奥博】

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Human CD6 Alexa Fluor 488 Affinity Purified PAb (100 TESTS)

货号： FAB627G 基本售价： 3955.9 元规格： 100 Tests

产品信息

概述
货号	FAB627G
别名	CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6
反应种属	Human
应用	Flow Cytometry(5 µL/10⁶cells)
目标/特异性	Detects recombinant human CD6 in direct ELISAs and Western blots.
使用方法	Flow Cytometry: 5 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	923 (Human); 12511 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CD318 is a ligand for CD6 Authors: G Enyindah-A, Y Li, JH Ruth, DS Spassov, KE Hebron, A Zijlstra, MM Moasser, B Wang, NG Singer, H Cui, RA Ohara, SM Rasmussen, DA Fox, F Lin Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human CD6 His18-Glu398 Accession # Q8WWJ7
生物活性	Human
标记	Alexa Fluor 488
背景	Human CD6 is a 105 kDa (unphosphorylated) -130 kDa(hyperphosphorylated) glycoprotein Group B member of the SRCR (ScavengerReceptor Cys-Rich) superfamily of molecules. It has a restricted expression pattern, being limited to select CD56^dimNK cells, B1a B cells (IgM producers), thymocytes and virtually all resting Tcells. Mature CD6 is a type Itransmembrane protein 651 amino acids (aa) in length. It contains a 385 aa extracellular region (aa19-402) that possesses three SRCR repeats, and a relatively long 245 aacytoplasmic domain. There are at leastsix alternative splice forms for human CD6, four of which (plus the full-lengthform) contain an unspliced extracellular region. The isoform used here for immunization(SwissProt: Q8WWJ7) is one of these four, showing splicing only in thecytoplasmic region. Thus, the polyclonalantibody used here will recognize all full-length CD6 molecules, and may, by anabsence of staining, suggest the presence of the two isoform variants that aremissing the third SRCR repeat that lies between aa 259-361. CD6 has multiple interaction partners. It complexes with CD5 in cis, contributing to the immunological synapse; in trans, it binds to dendritic cell CD166/ALCAMon the surface of adjacent cells. CD6 isalso known to bind soluble galectin-1 and -3, and to bind the PAMPs associatedwith pathogenic microbes. Notably, on T cells and following activation,full-length CD6 is downregulated, and a splice variant lacking the third SRCRis upregulated, abrogating any CD6:CD166 interaction. Functions attributed to CD6 include theinduction of chemokine secretion by NK cells, costimulation of antigenrecognition by T cells, and an antimicrobial activity attributed to an 85 kDasoluble form of CD6. Over aa 18-398,human and mouse CD6 share 68% aa sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Human CD Marker Products ITIM/ITAM Immunoreceptors and Related Molecules Leukocyte Receptors/Markers in Allergy and Asthma Scavenger Receptors T Cell Migration/Adhesion

参考图片

Detection of CD6 in Human Whole Blood Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Goat Anti-Human CD6 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB627G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins.