Human 5-Nucleotidase/CD73 Phycoerythrin MAb (Cl 606112) (100 TESTS)【科瑞奥博】

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Human 5-Nucleotidase/CD73 Phycoerythrin MAb (Cl 606112) (100 TESTS)

货号： FAB5795P 基本售价： 3955.9 元规格： 100 Tests

产品信息

概述
货号	FAB5795P
别名	5 nucleotidase (CD73); 5-NT; 5-NT; 5-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5-nucleotidase; eN; eNT; NT; NT55-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase
反应种属	Human
应用	Flow Cytometry
目标/特异性	Detects recombinant human 5-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5-Nucleotidase is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4907 (Human); 23959 (Mouse); 58813 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells Authors: SJ Li, Y Luo, LM Zhang, W Yang, GG Zhang Mol Med Rep, 2017;0(0):. Species: Human Sample Type: Whole Cells Application: Flow Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis. Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A Stem Cells Dev, 2014;23(5):541-54. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human 5‑Nucleotidase/CD73 Trp27-Ile511 Accession # P21589
生物活性	Human
标记	Phycoerythrin
背景	5-Nucleotidase (also [ecto]-5-nucleotidase/5-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3⁺ Treg lymphocytes, FDCs and B cells. 5-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5‑Nucleotidase shares 88% aa identity with both mouse and rat 5-Nucleotidase.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Lymphoblastic Leukemia Human CD Marker Products ICC / IHC Images: 5-Nucleotidase/CD73 Mesenchymal Stem Cell Markers Osteoblast and Osteoclast Markers Phosphatase-Coupled Sulfotransferase Assay Phosphatases and Regulators Regulatory T Cells (Tregs) Renal Markers

参考图片

Detection of 5‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human 5‑Nucleotidase/CD73 PE‑conjugated Monoclonal Antibody (Catalog # FAB5795P) or (B) Mouse IgG_2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.