货号 | FAB6561P |
别名 | activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; CD166 antigen; CD166; MEMDMGC71733 | 全称 | Activated Leukocyte Cell Adhesion Molecule |
反应种属 | Human |
应用 | Flow Cytometry |
目标/特异性 | Detects human ALCAM in Western blots. Shows approximately 50% cross-reactivity with recombinant mouse OCAM and no cross-reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1. |
使用方法 | Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 214 (Human); 11658 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Establishment of highly tumorigenic human colorectal cancer cell line (CR4) with properties of putative cancer stem cells. | |
纯化方式 | Protein A or G purified from ascites |
免疫原 | Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166 Trp28-Ala526 Accession # Q13740 |
生物活性 | Human |
标记 | Phycoerythrin |
背景 | ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of ALCAM/CD166 in Human Whole Blood Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Mouse Anti-Human ALCAM/CD166 PE‑conjugated Monoclonal Antibody (Catalog # FAB6561P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins. |