| 货号 | MAB9434-100 |
| 别名 | B2 bradykinin receptor; B2R; BDKRB2; BK-2 receptor; BK2; BK-2; BKR2; Bradykinin RB2; bradykinin receptor B2; BradykininRB2; BRB2; DKFZp686O088 | 全称 | Bradykinin Receptor B2 |
| 应用 | Western Blot,Immunohistochemistry |
| 目标/特异性 | Detects human RB2/BDKRB2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 624 (Human); 12062 (Mouse); 25245 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human RB2/BDKRB2 Met1-Gln391 Accession # P30411 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Bradykinin RB2 (BDKRB2) is a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. BDKRB2 expression is widespread in normal smooth muscle tissue and neurons. BDKRB2 associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. BDKRB2 forms a complex with PECAM1 and GNAQ and interacts with PECAM1. Aging cardiac endothelial cells gradually lose their capacity to express BDKRB2. This loss appears to be parallel with a loss of the angiogenic potential of the aging cells. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Bradykinin RB2/BDKRB2 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human Bradykinin RB2/BDKRB2. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB9434) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Bradykinin RB2/BDKRB2 at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Bradykinin RB2/BDKRB2 in Human Liver Cancer Tissue. Bradykinin RB2/BDKRB2 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB9434) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
