| 货号 | MAB3991-100 |
| 别名 | Anchorin CII; annexin A5; Annexin V; annexin-5; ANX5; ANXA5; ANXV; Calphobindin I; Endonexin II; ENX2CBP-I; Lipocortin V; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha |
| 应用 | Western Blot,Simple Western,Immunohistochemistry |
| 目标/特异性 | Detects human Annexin V in direct ELISAs. |
| 使用方法 | Western Blot: 0.25 µg/mL Simple Western: 2.5 µg/mL Immunohistochemistry: 5-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 308 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Annexin V Met1-Asp320 Accession # P08758 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Annexin V is a member of a calcium and phospholipid binding family of proteins with vascular anticoagulant activity. It is also known as PP4, PAP I, CPB-I, CaBP33, VACa, anchorin CII, lipocortin-V, endonexin II, and thromboplastin inhibitor. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Annexin V by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Annexin V Monoclonal Antibody (Catalog # MAB3991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Annexin V at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Annexin V in Human Placenta. Annexin V was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Annexin V Monoclonal Antibody (Catalog # MAB3991) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. | |
Detection of Human Annexin V by Simple WesternTM. Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Annexin V at approximately 39 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human Annexin V Monoclonal Antibody (Catalog # MAB3991). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
