| 货号 | IC4727R-100UG |
| 别名 | MAS-related GPR, member X2; mas-related G-protein coupled receptor member X2; MRGPRX2; MRGX2; MRGX2G protein-coupled receptor MRGX2 | 全称 | Mas-related G Protein-coupled Receptor Member X2 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human MRGX2. Stains human MRGX2 transfectants but not irrelevant transfectants. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 117194 (Human); 243978 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human MRGX2 Met1-Val330 Accession # Q96LB1 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 647 |
| 背景 | MRGX2 (MAS-related GPR, member X2) is a 330 amino acid (aa) G-protein coupled 7-transmembrane protein that is selectively expressed in small-diameter sensory neurons of dorsal root ganglia. Human MRGX shares 41‑52% aa identity with three other primate MRGX proteins, but has no ortholog in rodents. Binding of ligands such as cortistatin, proadrenomedullin peptides (PAMP-12 and –20) and basic peptides (substance P, neuropeptide Y) to MRGX2 can activate Gq or Gi regulated pathways. MRGX2 is thought to influence nociception and promote adrenal gland catecholamine secretion and IgE-independent mast cell degranulation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of MRGX2 in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with A) human MRGX2 or B) irrelevant transfectant and eGFP, was stained with Alexa Fluor® 647-conjugated Mouse Anti-Human MRGX2 Monoclonal Antibody (Catalog # IC4727R). Quadrant markers were set based on control antibody staining (Catalog # IC003R). View our protocol for Staining Membrane-associated Proteins. |
