| 货号 | IC2496U-100UG |
| 别名 | Abeta-degrading protease; EC 3.4.24; EC 3.4.24.56; FLJ35968; IDE; INSDEGM; Insulin protease; Insulinase; insulin-degrading enzyme; Insulysin | 全称 | Insulin Degrading Enzyme |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human Insulysin/IDE in direct ELISAs and Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3416 (Human); 15925 (Mouse); 25700 (Rat) |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Insulysin/IDE Met42-Leu1019 Accession # P14735 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 350 |
| 背景 | Insulysin, or insulin-degrading enzyme (IDE), is a zinc metallopeptidase of the inverzincin family. IDE is primarily located in the cytosol, but has been detected as a secreted enzyme and associated with the plasma membrane as well (1). The enzyme is expressed in many tissues, with the highest levels in liver, kidney, brain, and testis (2). IDE hydrolyzes a variety of regulatory peptides, including insulin, glucagon, atrial natriuretic factor, and transforming growth factor-alpha in vitro (1). In addition, IDE has been shown to degrade the amyloid beta (A beta ) peptide, which polymerizes into the plaques associated with Alzheimers disease (3). Deficiencies in IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus (DM2) and Alzheimers disease. The IDE region of human chromosome 10q has been genetically linked to DM2 (4). When the IDE gene was specifically disrupted in mice, IDE -/- animals developed hyperinsulinemia and glucose intolerance, characteristics of DM2 (5). The IDE -/- mice were also shown to have a significant decrease in A beta degradation in the brain, resulting in increased cerebral accumulation of A beta peptide. This in vivo evidence is consistent with the hypotheses that IDE is important for the degradation of insulin in cells and for the clearance of A beta peptide in the brain. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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