| 货号 | FAB8327V-100UG |
| 别名 | MGC120477; MNAT; monoamine neurotransmitter transporter; Monoamine transporter; SLC18A2; solute carrier family 18 (vesicular monoamine), member 2; Solute carrier family 18 member 2; SVAT; SVMT; SVMTMGC120478; synaptic vesicular amine transporter; VAT2; VAT2MGC26538; vesicle monoamine transporter type 2; vesicle monoamine/H+ antiporter; Vesicular amine transporter 2; VMAT2 | 全称 | Vesicular Monoamine Transporter 2/Solute Carrier Family 18, Member 2 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human VMAT2 in ELISAs and Western blots. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6571 (Human); 214084 (Mouse); 25549 (Rat) |
| 免疫原 | NS0 mouse myeloma cell line transfected with human VMAT2 Met1-Asp514 Accession # Q05940 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 405 |
| 背景 | The Vesicular Monoamine Transporter 2 (VMAT2), also known as VAT2 and SCL18A, is a 55-75 kDa member of the vesicular transporter family, a major facilitator superfamily. VMAT2 is a 12 transmembrane (TM) glycoprotein that is found in the membrane of brain neurosecretory vesicles. It transports monoamines (dopamine, serotonin, and particularly histamine) from the cytosol into secretion vesicles by exchanging two H+ ions for one molecule of amine. Human VMAT2 is 514 amino acids (aa) in length. It contains two cytoplasmic domains, a 20 aa and a 52 aa N- and C-terminal respectively, plus an extended 88 aa luminal loop between aa 42-129. There is one luminal, intrachain disulfide bond that contributes to amine transport (C126-C333). In addition, residues in TM domains 5-8 (aa 220-352) and 9-12 (aa 358‑462) also contribute to high affinity ligand interaction. VMAT2 is constitutively phosphorylated by CKII on S511 and S513. Within the cytoplasmic C-terminus, human VMAT2 is 94% aa identical to rat VMAT2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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