| 货号 | IC6035U-100UG |
| 别名 | F-box only protein 15; Fbox protein 15; F-box protein 15; FBX15; FBX15MGC39671; FBXO15 |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human F‑box protein 15/FBXO15 in direct ELISAs. In direct ELISAs, no cross-reactivitywith recombinant mouse F-box protein 15 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 201456 (Human); 361354 (Rat) |
| 免疫原 | E. coli-derived recombinant human F‑box protein 15/FBXO15 isoform 2 Asn298-Tyr434 Accession # Q8NCQ5-2 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 350 |
| 背景 | FBXO15 (F-box only protein 15; also FBX15) is a 50‑60 kDa member of the F-box family, O-subfamily of molecules that is most closely related to FBXO8 and O11. It is found in pluripotent stem cells and testis tissue, and apparently serves as a substrate recognition component of the SCF-type E3 ubiquitin ligase. Human FBXO15 (isoform 2 of accession Q8NCQ5) is 434 amino acids (aa) in length. It contains one F-box (aa 1‑41) that likely serves as an interaction domain for SKP1. There are two potential isoforms, one that contains an alternative start site 76 aa upstream of the standard site (isoform 1 of accession Q8NCQ5), and a second that shows complex splicing that includes a 66 aa insertion after Asn35, a Ser substitution for aa 186‑256, and a 106 aa substitution for aa 304‑346. Over aa 298‑434, human FBXO15 shares 53% aa identity with mouse FBXO15. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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