| 货号 | IC3220V-100UG |
| 别名 | bA268F1.1 (meprin A alpha (PABA peptide hydrolase)); EC 3.4.24; EC 3.4.24.18; Endopeptidase-2; MEP1A; meprin A subunit alpha; meprin A, alpha (PABA peptide hydrolase); Meprin alpha Subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha; PPHA |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MEP1B or recombinant mouse MEP1A is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4224 (Human); 17287 (Mouse); 25684 (Rat) |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Meprin alpha Subunit/MEP1A Val22-Gln601 Accession # AAA21338 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 405 |
| 背景 | Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid (aa) sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). Human Meprin alpha subunit consists of a signal peptide (aa 1 to 21), a pro region (aa 22 to 65), and a mature chain (aa 66 to 746) containing the following domains: catalytic (aa 62 to 263), MAM (aa 264 to 433), MATH (aa 434 to 593), EGF-like (aa 670 to 710), transmembrane (aa 713 to 740), and cytoplasmic (aa 741 to 746). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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