| 货号 | FAB21291T-100UG |
| 别名 | ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme | 全称 | TNF-alpha Converting Enzyme |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human TACE/ADAM17 Cytosolic Domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAM33 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6868 (Human); 11491 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human TACE/ADAM17 Asp695-Cys824 Accession # P78536 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 594 |
| 背景 | TACE (also known as ADAM17) is a type I membrane zinc protease of theADAM family that functions as a major sheddase on the cell surface. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of TACE/ADAM17 in HeLa Human Cell Lineby Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Alexa Fluor® 594-conjugatedMonoclonal Antibody (Catalog # FAB21291T, filled histogram) or isotype controlantibody (Catalog # IC002T, open histogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
