| 货号 | MAB27172-100 |
| 别名 | FLJ22667; hepatocellular carcinoma associated protein TB6; Hepatocellular carcinoma-associated protein TB6; MGC125361; MGC125362; pIgR; Poly-Ig receptor; polymeric immunoglobulin receptor | 全称 | Polymeric Immunoglobulin Receptor |
| 应用 | Blockade of Receptor-ligand Interaction |
| 目标/特异性 | Detects human pIgR in direct ELISAs. |
| 使用方法 | Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.1-0.6 ug/mL of Mouse anti-HumanpIgR will block 50% of the binding of 150 ng/mL of Human IgM toimmobilized Recombinant Human pIgR (Catalog # 2717-PG) coated at 2 ug/mL(100 µL/well). At 6 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Mouse IgG1 Clone # 825709 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5284 (Human); 18703 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human pIgR Lys19-Arg638 Accession # P01833 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The human Polymeric Immunoglobulin Receptor (pIgR; also known as Membrane Secretory Component) is a 100 kDa type I transmembrane glycoprotein that is synthesized as a 764 amino acid (aa) precursor. It includes a signal sequence (aa 1 - 18), an extracellular region (aa 19 - 638), a transmembrane segment (aa 639 - 661), and a cytoplasmic domain (aa 662 - 764) (1 - 3). The extracellular region consists of five Ig-like domains and a sixth non-Ig domain that connects to the membrane region. pIgR is expressed on secretory epithelial cells of exocrine tissues. Immunoglobulin isotypes consist of two heavy (H) and two light (L) chains. For IgA and IgM, this H2L2 monomer can form larger polymers through association with a joining chain (J chain). The Fc regions of IgA and IgM have a carboxy-terminal extension called a secretory tailpiece that binds the J chain (4). pIgR functions as a carrier that transports IgA and IgM across epithelium (5). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via a docking site on the J chain. This initiates a rearrangement in which a disulfide bond forms between pIgR and an IgA heavy chain (2). The complexes are then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is now generated by proteolytic cleavage of the sixth extracellular domain of pIgR and released into the lumen (6). This IgA-bound and proteolytically generated pIgR fragment is referred to as secretory component (SC). Notably, human pIgR transcytoses constitutively, with or without ligand, creating both bound and free, 78 kDa SC following cleavage (3). The extracellular region of pIgR is 64%, 65% and 70% aa identical to the equivalent region in rat, mouse and porcine, respectively. The receptor component of the complex anchors the SIgA molecule to mucous (7). SIgA is a crucial component of the mucosal immune system serving to protect the large expanse of mucous membranes that form a barrier between the interior of the body and the external environment (8). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
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