性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3673 (Human); 16398 (Mouse) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
PLoS ONE, 2012;7(10):e47187.
Species: Human
Sample Type: Whole Cells
Application: Flow |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human keratinocytes |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Takada, Y. et al. (2007) Genome Biol. 8:215.
- Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
- Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
- Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
- McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
- Sarratt, K.L. et al. (2005) Blood 106:1268.
- Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
- Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
- McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
- Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
- Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
- Edelson, B.T. et al. 2006) Blood 107:143.
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