概述| 货号 | FAB1934P |
| 别名 | CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of |
全称 | Density Enhanced Receptor Protein Tyrosine Phosphatase | | 反应种属 | Human |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human DEP-1/CD148 in Western blots. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 5795 (Human); 19271 (Mouse); 29645 (Rat) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
Immunol. Lett., 2011;134(2):113-21.
Species: Human
Sample Type: Whole Cells
Application: Flow
Regulation of Src Family Kinases Involved in T Cell Receptor Signaling by Protein-tyrosine Phosphatase CD148.
Authors: Stepanek O, Kalina T, Draber P
J. Biol. Chem., 2011;286(25):22101-12.
Species: Human
Sample Type: Whole Cells
Application: Flow |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Phytohemagglutinin-stimulated human peripheral blood mononuclear cells |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP Receptor Type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-Catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
- Sorby, M. et al. (2001) Oncogene 20:5219.
- Jandt, E. et al. (2003) Oncogene 22:4175.
- Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
- Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
- Borges, L.G. et al. (1996) Circ. Res. 79:570.
- de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
- Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
- Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
- Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
- Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
- Holsinger, L.J. et al. (2002) Oncogene 21:7067.
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参考图片Detection of DEP‑1/CD148 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human DEP‑1/CD148 PE‑conjugated Monoclonal Antibody (Catalog # FAB1934P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
