Human CD14 Phycoerythrin MAb (Clone 134620) (100 TESTS)【科瑞奥博】

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Human CD14 Phycoerythrin MAb (Clone 134620) (100 TESTS)

货号： FAB3832P-100 基本售价： 3668.3 元规格： 100 Tests

产品信息

概述
货号	FAB3832P-100
别名	CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
反应种属	Human
应用	Flow Cytometry
目标/特异性	Detects human CD14 in direct ELISAs and Western blots.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CXCR4 positive and angiogenic monocytes in myocardial infarction. Thromb Haemost, 2013;109(2):255-62. Species: Human Sample Type: Whole Blood Application: Flow Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium. Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G PLoS ONE, 2012;7(10):e47187. Species: Human Sample Type: Whole Cells Application: Flow Human mesenchymal stromal cells express CD14 cross-reactive epitopes. Authors: Pilz GA, Braun J, Ulrich C, Felka T, Warstat K, Ruh M, Schewe B, Abele H, Larbi A, Aicher WK Cytometry A, 2011;79(8):635-45. Species: Human Sample Type: Whole Cells Application: Flow Epitope specificity and clonality of EBV-specific CTLs used to treat posttransplant lymphoproliferative disease. Authors: McAulay KA, Haque T, Urquhart G, Bellamy C, Guiretti D, Crawford DH J. Immunol., 2009;182(6):3892-901. Species: Human Sample Type: Whole Cells Application: Flow Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways. Authors: Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J J. Mol. Biol., 2006;367(1):25-35. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human CD14 Thr20-Cys352 Accession # P08571
生物活性	Human
标记	Phycoerythrin
背景	CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.
运输条件	Blue Ice
存放说明	4℃
参考文献	Wright, S.D.et al. (1990) Science 249:1431. Pugin, J.et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744. Beutler, B. (2000) Current Opinion in Immunology 12:20. Stelter, F. (2000) Chem. Immunol. 74:25.

参考图片

Detection of CD14 in Human PBMCs byFlow Cytometry. Human peripheral blood mononuclearcells (PBMCs) were stained with Mouse Anti-Human CD14 PE‑conjugatedMonoclonal Antibody (Catalog # FAB3832P). View our protocol for StainingMembrane-associated Proteins.