| 货号 | IC2890F |
| 别名 | interferon alpha induced transcriptional activator; ISGF-3; MGC59816; P113; signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kD; signal transducer and activator of transcription 2, 113kDa; STAT113 | 全称 | Signal Transducer and Activator of Transcription 2 |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human STAT2 when phosphorylated at Y689 in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6773 (Human); 20847 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing the human STAT2 Y689 site |
| 生物活性 | Human |
| 标记 | Fluorescein |
| 背景 | STAT2 (Signal Transducer and Activator of Transcription #2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with Type I ( alpha - and beta -) Interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y689 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Phospho-STAT2 (Y689) in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitts lymphoma cell line untreated (light orange filled histogram) or treated with Recombinant Human IFN‑ alpha A (Catalog # 11100-1) (dark orange filled histogram) was stained with Rabbit Anti-Human Phospho-STAT2 (Y689) Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2890F) or isotype control antibody (Catalog # IC105F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
