Human TGF-beta 1 Fluorescein MAb (Clone 9016) (100 TESTS)
货号：
IC240F 基本售价：
3529.5 元 规格：
100 Tests
产品信息
概述| 货号 | IC240F |
| 别名 | CEDLAP; DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1 |
全称 | Transforming Growth Factor beta 1 | | 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells)
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| 目标/特异性 | Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
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| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells.
Authors: Ansa-Addo EA, Lange S, Stratton D, Antwi-Baffour S, Cestari I, Ramirez MI, McCrossan MV, Inal JM
J. Immunol., 2010;185(9):5236-46.
Species: Human
Sample Type: Whole Cells
Application: Flow |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1 |
| 生物活性 | Human |
| 标记 | Fluorescein |
| 背景 | TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Airway Inflammation and Remodeling
- Cancer Biomarkers
- Cytokines and Receptors in Allergy and Asthma
- Cytokines and Receptors in Angiogenesis
- Early Mesodermal Lineage Markers
- EMT Induction
- HIF Transcription Factors
- ICC / IHC Images: TGF-beta 1
- Inflammatory Mediators
- Myeloid-derived Suppressor Cells (MDSC)
- Platelets
- Preeclampsia
- Regulatory T Cells (Tregs)
- TGF-beta Family
- TGF-beta Family Ligands
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参考图片Detection of TGF‑ beta 1 in PC‑3 human prostate cancer cell line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human TGF‑ beta 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC240F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
