| 货号 | FAB7446G |
| 别名 | CD1; CD1A; CD1b antigen; CD1B antigen, b polypeptide; CD1b molecule; CD1b; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3; MGC125990; MGC125991; R1; T-cell surface glycoprotein CD1b |
| 反应种属 | Human |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human CD1b in direct ELISAs.In direct ELISAs, no cross-reactivitywith recombinant human (rh) CD1a, rhCD1c, rhCD1d, rhCD1e, recombinant mouse (rm)CD1d1, or rmCD1d2 is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 910 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CD1b Ser18-Ser303 Accession # P29016 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | CD1b is a 44-46 kDa member of the CD1 family of proteins. It is one of five CD1 isoforms (CD1a-e) that resemble MHC-I type molecules. Based on structure, CD1a, 1b and 1c can be grouped together, with CD1d and 1e existing as stand-alone forms. CD1b is a 316 amino acid (aa) type I transmembrane glycoprotein that contains one Ig-like domain in its ECD. It is expressed in the late endosome/lysosome compartment of myeloid-type dendritic cells. Following synthesis, CD1b associates with SAP-C, binds two short lipids in an unusually large ligand-binding groove, and transits either to the plasma membrane, or more commonly, the endosomal compartment. In either case, exposure to Gm- lipid A or mycobacterial glyolipid results in a substitution of the foreign lipid for the two short-chain self lipids, followed by its presentation on the plasma membrane. Select alpha beta and gamma δ T cell recognize these lipids and typically repond with Th1-type cytokines (IFN-gamma and TNF-alpha ). Unlike other CD1 family molecules, CD1b does not seem to have a soluble form. There is no rodent counterpart to human CD1b, only to CD1d. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD1b in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1b Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7446G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
