Human MMP-9 Fluorescein MAb (Clone 56129) (100 TESTS)
货号：
IC9111F 基本售价：
3529.5 元 规格：
100 Tests
产品信息
概述| 货号 | IC9111F |
| 别名 | 92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP-9; type V collagenase |
全称 | Matrix Metalloproteinase 9 | | 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects human MMP-9 in Flow Cytometry. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 4318 (Human); 17395 (Mouse); 81687 (Rat) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. JAK/STAT regulation of Aspergillus fumigatus corneal infections and IL-6/23-stimulated neutrophil, IL-17, elastase, and MMP9 activity
Authors: PR Taylor, S Roy, EC Meszaros, Y Sun, SJ Howell, CJ Malemud, E Pearlman
J Leukoc Biol, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Flow
Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
Eur. J. Haematol., 2010;84(3):239-51.
Species: Human
Sample Type: Whole Cells
Application: Flow |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human MMP-9 |
| 生物活性 | Human |
| 标记 | Fluorescein |
| 背景 | Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Airway Inflammation and Remodeling
- APP Cleavage and Amyloid-beta Degradation
- Cancer Biomarkers
- Cell to Cell Adhesion Disassembly During EMT
- Collagen Modifying Enzymes
- Extracellular Matrix Remodeling and Cell Migration During EMT
- Gametes and Fertilization
- ICC / IHC Images: MMP-9
- Inflammatory Mediators
- Mesenchymal Cells and EMT
- Metalloproteases and Regulators
- MMP Modulation of Cytokines
- MMP-12 in Smoking-Related Pulmonary Disease
- MMPs, TIMPs and Related Molecules
- New Tools: Active MMP Activity Assays
- OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression
- Preeclampsia
|
参考图片Detection of MMP‑9 in NS0 Mouse Cell Line Transfected with Human MMP-9 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human MMP-9 was stained with Mouse Anti-Human MMP‑9 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC9111F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
