Human LAMP-1/CD107a Phycoerythrin MAb (Clone 508921) (100 TESTS)【科瑞奥博】

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Human LAMP-1/CD107a Phycoerythrin MAb (Clone 508921) (100 TESTS)

货号： IC4800P 基本售价： 3529.5 元规格： 100 Tests

产品信息

概述
货号	IC4800P
别名	CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1
全称	Lysosome-associated Membrane Glycoprotein 1
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human LAMP1/CD107a in direct ELISAs and Western blots.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3916 (Human); 16783 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J Lab. Invest., 2012;92(7):967-77. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a Ala28-Asn380 Accession # P11279
生物活性	Human
标记	Phycoerythrin
背景	Lysosome-associatedmembraneprotein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.
运输条件	Blue Ice
存放说明	4℃
参考文献	Granulocyte Markers Human CD Marker Products ICC / IHC Images: LAMP-1/CD107a Isolation, Culture, and Identification of Natural Killer Cells Lysosomal Enzymes Myeloid Lineage Markers

参考图片

Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry. Enriched human NK cells either (A) treated with 1:1 ratio with K562 cell line and 3 uM monensin for 5 hours or (B) untreated were stained with Mouse Anti-Human LAMP‑1/CD107a PE‑conjugated Monoclonal Antibody (Catalog # IC4800P) and Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of LAMP‑1/CD107a in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP‑1/CD107a PE‑conjugated Monoclonal Antibody (Catalog # IC4800P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.