Human B7-1/CD80 Phycoerythrin MAb (Clone 37711) (100 TESTS)【科瑞奥博】

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Human B7-1/CD80 Phycoerythrin MAb (Clone 37711) (100 TESTS)

货号： FAB140P-100 基本售价： 3529.5 元规格： 100 Tests

产品信息

概述
货号	FAB140P-100
别名	Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80
反应种属	Human
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ChemR23, the receptor for chemerin and resolvin E1, is expressed and functional on M1 but not on M2 macrophages. Authors: Herova M, Schmid M, Gemperle C, Hersberger M J Immunol, 2015;194(5):2330-7. Species: Human Sample Type: Whole Cells Application: Flow Critical role of lipid rafts in CD154-mediated T cell signaling. Authors: El Fakhry Y, Alturaihi H, Diallo D, Merhi Y, Mourad W Eur. J. Immunol., 2010;40(3):770-9. Species: Human Sample Type: Whole Cells Application: Flow Eosinophils induce DC maturation, regulating immunity. Authors: Lotfi R, Lotze MT J. Leukoc. Biol., 2007;83(3):456-60. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80 Val35-Asn242 (predicted) Accession # P33681
生物活性	Human
标记	Phycoerythrin
背景	B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequence identity. Human and mouse B7-1 share 44% aa sequence identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.
运输条件	Blue Ice
存放说明	4℃
参考文献	Azuma, M.et al. (1993) Nature 366:76. Freeman, G.J.et al. (1993) Science 262:909. Freeman, G.et al. (1991) J. Exp. Med. 174:625. Selvakumar, A.et al. (1993) Immunogenetics 38:292. Chen, C.et al. (1994) J. Immunol. 152:4929. Freeman, G.J.et al. (1993) J. Exp. Med. 178:2185.

参考图片

Detection of B7‑1/CD80 in Raji Human Burkitts Cell Line by Flow Cytometry. Raji human Burkitts lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 PE‑conjugated Monoclonal Antibody (Catalog # FAB140P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.