Human Granzyme A Fluorescein MAb (Clone 356412) (100 TESTS)【科瑞奥博】

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Human Granzyme A Fluorescein MAb (Clone 356412) (100 TESTS)

货号： IC29051F 基本售价： 3529.5 元规格： 100 Tests

产品信息

概述
货号	IC29051F
别名	CTL Tryptase; CTLA3; CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease); Cytotoxic T-lymphocyte proteinase 1; EC 3.4.21; Fragmentin-1; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); granzyme A; Granzyme-1; GZMA; H factor; Hanukkah factor; HF; HFSP; HFSPEC 3.4.21.78
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human Granzyme A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Granzyme B, C, D, G, or H is observed.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3001 (Human); 14938 (Mouse); 266708 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. MAIT cells are licensed through granzyme exchange to kill bacterially sensitized targets. Authors: Kurioka, A, Ussher, J E, Cosgrove, C, Clough, C, Fergusson, J R, Smith, K, Kang, Y-H, Walker, L J, Hansen, T H, Willberg, C B, Klenerman, P Mucosal Immunol, 2015;8(2):429-40. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Granzyme A Cys26-Val262 Accession # P12544
生物活性	Human
标记	Fluorescein
背景	Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin (1). Human Granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1‑26), a propeptide (residues 27‑28) and a mature chain (residues 29‑262) (2). The purified recombinant human Granzyme A consists of residues 26 to 262. After being activated by Lysyl Endopeptidase, it cleaves a thioester substrate.
运输条件	Blue Ice
存放说明	4℃
参考文献	Lieberman, J. and Z. Fan (2003) Curr. Opin. Immunol. 15:553. Gershenfeld, H.K. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1184.

参考图片

Detection of Granzyme A in CD56⁺ Human Blood Cells by Flow Cytometry.CD56⁺ human whole blood cells were stained with Mouse Anti-Human Granzyme A Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC29051F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.