Human IL-27 Fluorescein MAb (Clone 307426) (100 TESTS)
货号：
IC25261F 基本售价：
3529.5 元 规格：
100 Tests
产品信息
概述| 货号 | IC25261F |
| 别名 | IL-27 p28 subunit; IL-27 subunit alpha; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A |
全称 | Interleukin 27 | | 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects human IL-27 in direct ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 246778 (Human); 246779 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IL‑27
Arg21-Lys229 of EBI-3 (Accession #Q14213.2) and Phe29-Pro243 of p28 (Accession #AAM34498) |
| 生物活性 | Human |
| 标记 | Fluorescein |
| 背景 | IL-27 is anon-covalent heterodimeric molecule that belongs to the IL-6/IL-12 family oflong type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a33-34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23,and p28, the 28-30 kDa glycoprotein that is related to the p35 chain ofIL-12. The human EBI3 gene encodes a 229 amino acid (aa) precursor thatcontains a 20 aa signal peptide and 209 aa mature protein. The mature regioncontains two potential N-linked glycosylation sites, two fibronectin type IIIdomains, and two pairs of conserved cysteine residues with a WSXWS-like motifthat places the molecule in the hematopoietin receptor family. Although p40,the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidenceto date that EBI3 also homodimerizes.However, EBI3 is known to heterodimerizewith the p35 subunit of IL-12, generating the cytokine IL-35. Human EBI3 is 61%aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor thatcontains a 28 aa signal sequence and 215 aa mature region. The matureregion is characterized by the presence of four alpha -helices, placing it in theIL-6 family of helical cytokines. Human p28 is 70% aa identical to mouse p28.IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27binds to and signals through a heterodimeric receptor complex composed of WSX-1(TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. p28 alsoheterodimerizes with CLF1 and signals through the IL-6R:gp130 complex. IL-27has both anti- and proinflammatory properties. As an anti‑inflammatory, IL-27seems to induce a general negative feedback program that limits T and NK-T cellactivity. At the onset of infection, IL-27 induces an IL‑12 receptor onnaïve CD4+ T cells, making them susceptible to subsequent IL-12activity that generates Th1 cells at the expense of Th2 and Th17 cells.Finally, IL-27 upregulates both MHC-II and chemokine (CXCL9; CXCL10) expressionon vascular endothelium, suggesting a role for IL-27 in vascular inflammation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - IL-12 Family
- IL-27: Balancing T Cell-Mediated Immune Responses
- Interactive Pathway: Th1 Differentiation
- Isolation, Culture, and Identification of Natural Killer Cells
- Multiple Sclerosis and Myelin Related Molecules
- Th1 Cells
- The IL-12 Family of Cytokines & Mechanisms of Intestinal Inflammation
|
参考图片 Detection of IL‑27 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) either (A) untreated (light orange filled histogram) or (B) treated with PMA and Recombinant Human IL‑2 (Catalog # 202-IL, dark orange filled histogram) were stained with Mouse Anti-Human IL‑27 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC25261F) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
