货号 | IC13292F |
别名 | beta 1 (88kD); beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923 |
反应种属 | Human |
应用 | Intracellular Staining by Flow Cytometry |
目标/特异性 | Detects human, mouse, and rat beta -Catenin in Western blots. |
使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 1499 (Human); 12387 (Mouse); 84353 (Rat) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human beta -Catenin Ala2-Leu781 Accession # P35222 |
生物活性 | Human |
标记 | Fluorescein |
背景 | beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of beta ‑Catenin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human beta ‑Catenin Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC13292F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |