| 货号 | FAB10721F |
| 别名 | Siglec-5/Siglec-14 | 全称 | Sialic Acid Binding Ig-like Lectin 5 |
| 反应种属 | Human |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human Siglec‑5 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8778 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Siglec-5 and Siglec-14 are polymorphic paired receptors that modulate neutrophil and amnion signaling responses to group B Streptococcus. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Siglec‑5 Lys18-Thr434 Accession # O15389 |
| 生物活性 | Human |
| 标记 | Fluorescein |
| 背景 | Siglecs (Sialic acid binding Ig-like Lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5, also known as CD170, binds to alpha-2,3- and alpha-2,6-linked sialic acid with similar affinity. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in monocytes and neutrophils. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Siglec‑5/Siglec‑14 in Human Monocytes by Flow Cytometry. Human monocytes were stained with Mouse Anti-Human Siglec‑5/Siglec‑14 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB10721F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins. |
