Human IL-12/IL-23 p40 Fluorescein MAb (Clone 169503) (100 TESTS)【科瑞奥博】

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Human IL-12/IL-23 p40 Fluorescein MAb (Clone 169503) (100 TESTS)

货号： IC6092F 基本售价： 3529.5 元规格： 100 Tests

产品信息

概述
货号	IC6092F
别名	CLMF2; IL12B; IL-12B; NKSF2
全称	Interleukin 12/Interleukin 23 p40
反应种属	Human
应用	Intracellular Staining by Flow Cytometry
目标/特异性	Detects Human IL‑12/IL‑23 p40 in flow cytometry.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3593 (Human); 16160 (Mouse); 64546 (Rat); 403976 (Canine); 493741 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation. Authors: Napoletano C, Zizzari IG, Rughetti A, Rahimi H, Irimura T, Clausen H, Wandall HH, Belleudi F, Bellati F, Pierelli L, Frati L, Nuti M Eur. J. Immunol., 2012;42(4):936-45. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑23 p19/p40 heterodimer Ile23-Ser328 (IL‑12/IL‑23 p40 subunit) Accession # P29460
生物活性	Human
标记	Fluorescein
背景	Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8^- and CD8⁺ subtypes, however only IL-23 can act directly on CD8⁺ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis gamma delta T Cells IL-10 and IL-12 Influence in Dendritic Cell Maturation IL-12 Family IL-12: Cell Protection from UVB Th1 Cells Th17 Cells

参考图片

Detection of IL‑12/IL‑23 p40 in Mature Dendritic Cells by Flow Cytometry. Mature dendritic cells were stained with Mouse Anti-Human IL‑12/IL‑23 p40 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC6092F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.