| 货号 | IC4800G |
| 别名 | CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1 | 全称 | Lysosome-associated Membrane Glycoprotein 1 |
| 反应种属 | Human |
| 应用 | Flow Cytometry,Immunocytochemistry |
| 目标/特异性 | Designed to visualize the expression of LAMP-1 by fluorescence microscopy for staining cells and tissues. Conjugated LAMP-1 antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. The unconjugated antibody detects human LAMP-1/CD107a in direct ELISAs and Western blots. |
| 使用方法 | Flow Cytometry: 5 µL/106cells Immunocytochemistry: 1:10 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3916 (Human); 16783 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human LAMP-1/CD107a Ala28-Asn380 Accession # P11279 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | Lysosome-associatedmembraneprotein-1 (LAMP-1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP-2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP-1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP-1 shares 64% aa identity with mouse LAMP-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry. Human NK Cells either (A) stimulated in a 1:1 ratio with K562 human chronic myelogenous leukemia cell line and 3 μM monensin for 5 hours or (B) ustimulated were stained with Mouse Anti-Human LAMP‑1/CD107a Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4800G) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins. | |
LAMP1/CD107a in HeLa Human Cell Line. LAMP1/CD107a was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LAMP1/CD107a Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4800G) at 1:10 dilution for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
