Human PDGF R beta Fluorescein MAb (Clone # PR7212) (100 TESTS)
货号：
FAB1263F 基本售价：
3529.5 元 规格：
100 Tests
产品信息
概述| 货号 | FAB1263F |
| 别名 | beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide |
全称 | Platelet-derived Growth Factor Receptor beta | | 反应种属 | Human |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor beta -subunit complexed with 125I‑PDGF-BB (3). It does not recognize the PDGF receptor alpha ‑subunit. Detects the PDGF receptor beta -subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 5159 (Human); 18596 (Mouse); 24629 (Rat) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
Stem Cells, 2015;33(1):133-45.
Species: Human
Sample Type: Whole Cells
Application: Flow
The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
Exp. Cell Res., 2012;318(4):371-8.
Species: Human
Sample Type: Whole Cells
Application: Flow |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | Human skin fibroblast membrane extracts |
| 生物活性 | Human |
| 标记 | Fluorescein |
| 背景 | PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immuno‑globulinlike domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Hartet al. (1987) J. Biol. Chem. 262:10780.
- Gronwaldet al. (1988) Proc. Natl. Acad. Sci. 85:3435.
- Seifertet al. (1989) J. Biol. Chem. 264:8771.
- Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
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参考图片Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R beta Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1263F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins. |
