Human DNAM-1/CD226 Fluorescein MAb (Clone 102511) (100 TESTS)【科瑞奥博】

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Human DNAM-1/CD226 Fluorescein MAb (Clone 102511) (100 TESTS)

货号： FAB666F 基本售价： 3529.5 元规格： 100 Tests

产品信息

概述
货号	FAB666F
别名	CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1
全称	DNAX Accessory Molecule 1
反应种属	Human
应用	Flow Cytometry
目标/特异性	Detects human DNAM-1 in direct ELISAs and Western blots.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Crystal Structure of Cell Adhesion Molecule Nectin-2/CD112 and Its Binding to Immune Receptor DNAM-1/CD226. Authors: Liu J, Qian X, Chen Z, Xu X, Gao F, Zhang S, Zhang R, Qi J, Gao GF, Yan J J. Immunol., 2012;188(11):5511-20. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	Mouse myeloma cell line NS0-derived recombinant human DNAM-1 Glu19-Asn247 (predicted) Accession # Q15762
生物活性	Human
标记	Fluorescein
背景	DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8⁺ T cells, and mast cells (2‑6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the forrmation of osteoclasts (7‑10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12‑14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359. Shibuya, A. et al. (1996) Immunity 4:573. Bottino, C. et al. (2003) J. Exp. Med. 198:557. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533. Dardalhon, V. et al. (2005) J. Immunol. 175:1558. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190. Reymond, N. et al. (2004) J. Exp. Med. 199:1331. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491. Morimoto, K. et al. (2007) Oncogene July 16 epub. Shibuya, K. et al. (1999) Immunity 11:615. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829. Shibuya, A. et al. (1998) J. Immunol. 166:1671.

参考图片

Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3^- cells were stained with Mouse Anti-Human DNAM-1/CD226 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB666F) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.