Human IL-13 R alpha 2 Fluorescein Affinity Purified PAb (100 TESTS)【科瑞奥博】

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Human IL-13 R alpha 2 Fluorescein Affinity Purified PAb (100 TESTS)

货号： FAB614F 基本售价： 3390.7 元规格： 100 Tests

产品信息

概述
货号	FAB614F
别名	cancer/testis antigen 19; CD213a2 antigen; CD213a2; CT19; IL-13 receptor subunit alpha-2; IL13BP; IL-13R subunit alpha-2; IL13R; IL-13R; IL13RA2; IL-13RA2; IL-13R-alpha-2; interleukin 13 binding protein; interleukin 13 receptor alpha 2 chain; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein
全称	Interleukin 13 Receptor alpha 2
反应种属	Human
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse IL-13 R alpha 2 is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3598 (Human); 16165 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Absence of a role for interleukin-13 in inflammatory bowel disease. Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T Eur J Immunol, 2014;44(2):370-85. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 2 Cys22-Leu342 Accession # Q14627
生物活性	Human
标记	Fluorescein
背景	Two type1 membrane proteins belonging to the Hemopoietin Receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha , is now referred to as IL-13 R alpha 2. Human IL-13 R alpha 2 was originally cloned from the Caki-1 human renal carcinoma cell line. The IL-13 R alpha 2 cDNA encodes a 380 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide, a 317 residue extracellular domain, a 20 aa residue transmembrane region and a 17 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the 17 aa residue cytoplasmic domain of IL-13 R alpha 2 is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with the IL-4 R to form a high-affinity receptor complex capable of transducing an IL‑13‑dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 aa residues of the human and mouse IL‑13 R alpha 2 are nearly identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine.
运输条件	Blue Ice
存放说明	4℃
参考文献	Caput, D. et al. (1996) J. Biol. Chem. 271:16921. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317. Aman, M.J.et al. (1996) J. Biol. Chem. 271:29265. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.

参考图片

Detection of IL‑13 R alpha 2 in A375 Human Cell Line by Flow Cytometry. A375 human melanoma cell line was stained with Goat Anti-Human IL‑13 R alpha 2 Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB614F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.