Human Fibronectin Fluorescein MAb (Clone P1H11) (100 TESTS)
货号：
IC1918F 基本售价：
3390.7 元 规格：
100 Tests
产品信息
概述| 货号 | IC1918F |
| 别名 | FN1 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects human Fibronectin in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2335 (Human); 280794 (Bovine) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human plasma-derived Fibronectin |
| 生物活性 | Human |
| 标记 | Fluorescein |
| 背景 | Human Fibronectin (FN) is a 210-220 kDa extracellular matrix protein that serves as a cornerstone for cell-matrix interactions. It is a sulfated glycoprotein 2055 amino acids (aa) in length. Along its length, it exhibits three types of repeating modules; twelve type I, two type II and 16 type III modules. There are more than 15 alternative splice variants, most in the C-terminal half of the molecule. FN circulates as a disulfide-linked homodimer, a product of hepatocytes. It also exists as a very large covalent multimer, likely the product of a wide variety of cell types. Within these multimers will be found FN-Fibrinogen, -Collagen 13 and -Mac2BP complexes. The multidomain nature of FN allows for binding of Fibrin, GAGs (HS and CS), Syndecans and Integrins alpha 5 beta 1 and alpha V beta 3. Over aa 31-2386 (full-length), human FN shares 91% aa sequence identity with mouse FN.
|
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Coating Substrates for NSCs
- Extracellular Matrix and Related Molecules
- Extracellular Matrix Molecules
- ICC / IHC Images: Fibronectin
- Mesenchymal Cells and EMT
- Mesenchymal Stem Cell Markers
- Osteoblast and Osteoclast Markers
- Osteoblast Markers
- Osteocyte Markers
- Osteogenesis Markers
- Preeclampsia
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
|
参考图片Detection of Fibronectin in WS‑1 Human Cell Line by Flow Cytometry. WS‑1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1918F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
