Human IL-1 beta/IL-1F2 Fluorescein MAb (Clone 8516) (100 TESTS)【科瑞奥博】

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Human IL-1 beta/IL-1F2 Fluorescein MAb (Clone 8516) (100 TESTS)

货号： IC201F 基本售价： 3390.7 元规格： 100 Tests

产品信息

概述
货号	IC201F
别名	catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta /IL-1F2 and recombinant porcine IL-1 beta /IL-1F2 and no cross-reactivity with recombinant rat (rr) IL-1 beta /IL-1F2, rmIL‑1 alpha, recombinant human IL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Kinin receptor expression during Staphylococcus aureus infection. Authors: Bengtson SH, Phagoo SB, Norrby-Teglund A, Pahlman L, Morgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H Blood, 2006;108(6):2055-63. Species: Human Sample Type: Whole Cells Application: Flow Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells. Authors: Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM FEBS Lett., 2003;538(1):149-54. Species: Human Sample Type: Whole Cells Application: Flow T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta. Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM J. Allergy Clin. Immunol., 2002;110(4):624-33. Species: Human Sample Type: Whole Cells Application: Flow Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist. Authors: Vamvakopoulos J, Green C, Metcalfe S Eur. J. Immunol., 2002;32(10):2988-96. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IL-1 beta /IL-1F2 Ala117-Ser269 Accession # P01584
生物活性	Human
标记	Fluorescein
背景	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R Accessory Protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21. March, C.J. et al. (1985) Nature 315:641. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. USA 81:7907. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

参考图片

Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with (A) Mouse Anti-Human IL‑1 beta /IL‑1F2 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC201F) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). Excess Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) inhibits IC201F staining (B). Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.