货号 | IC208F |
别名 | 3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 | 全称 | Interleukin 8 |
反应种属 | Human |
应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects human CXCL8/IL‑8 in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant porcine CXCL8/IL‑8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 beta. |
使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human CXCL8/IL‑8 Ser28-Ser99 Accession # P10145 |
生物活性 | Human |
标记 | Fluorescein |
背景 | CXCL8/IL-8 is an 8-9 kDa member of the CXCL subfamily of chemokines. It was originally discovered and purifiedindependently by a number of laboratories as a neutrophil chemotactic andactivating factor. It has also referred to as Neutrophil Activating Protein(NAP), Monocyte-derived Neutrophil Chemotactic Factor (MDNCF) and granulocytechemotactic protein (GCP). Many cell types, including monocyte/macrophages, Tcells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes,chondrocytes, and mammary plus alveolar epithelium can produce CXCL8 inresponse to a wide variety of stimuli, such as IL-1b, TNF-a, LPS, MRP-8/14 and viruses. When secreted, it circulates asboth a monomer and dimer, with the dimeric forms being a homodimer andheterodimer with CXCL4/PF4. CXCL8 bindsto CXCR-1 and -2, with the CXCL8 monomer favoring CXCR-1, and the CXCL8homodimer favoring CXCR-2 and glycosaminoglycans. CXCL8 is a potentchemoattractant for neutrophils. In addition, CXCL8 also has a wide range ofother pro-inflammatory effects. CXCL8 causes degranulation of neutrophilspecific granules and azurophilic granules. It also induces expression of thecell adhesion molecules CD11/CD18, and enhances the adherence of neutrophils toendothelial cells and sub-endothelial matrix proteins. Besides neutrophils,CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 hasbeen reported to be a co-mitogen for keratinocytes, and has also shown to be anautocrine growth factor for melanoma cells. CXCL8 is also reported to beangiogenic both in vivoandin vitro. There is no directstructural rodent counterpart to human IL-8. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of CXCL8/IL‑8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL‑8 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC208F) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P), as shown in panel A.Inhibition of IC208F staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |